polymerization reaction buffer  (New England Biolabs)

Journal: International Journal of Molecular Sciences

Article Title: Measurement and Characterization of the Electrical Properties of Actin Filaments

doi: 10.3390/ijms25105485

Figure Lengend Snippet: Standard polymerization reactions of samples of actin at different concentrations: 0.1 mg/mL and 0.35 mg/mL. A control solution without the addition of actin polymerization buffer has been analyzed as well. ( A ) Data obtained; ( B ) data smoothed with FFT windows of 4 points.

Article Snippet: Then, the actin solution was aliquoted in 100 µL samples, and the polymerization reaction was assembled by adding 1 10 th of the volume of actin polymerization buffer (cytoskeleton, APB at 10X Cat. # BSA02).

Techniques:

Journal: Science Advances

Article Title: Scalable acoustic virtual stirrer for enhanced interfacial enzymatic nucleic acid reactions

doi: 10.1126/sciadv.adt6955

Figure Lengend Snippet: ( A ) Schematic diagrams depicting the polymerization catalyzed by DNA polymerase I, the ligation catalyzed by T4 DNA ligase and the digestion catalyzed by DNase I, along with comparisons of interfacial reaction efficiency when using AVS versus not using AVS. ( B ) Schematic diagram outlining the iEOS process on AVS. ( C ) Comparison of the stepwise yield, full-length yield, and deletion error rate with and without the use of AVS. ( D ) On the basis of NGS results, a synthetic step-by-step yield was determined through comparison with the target sequence (* P < 0.05; ** P ≤ 0.001; *** P ≤ 0.0001).

Article Snippet: Following this, a 10-μl polymerization reaction buffer was prepared by mixing Klenow fragment of DNA polymerase (1 U/μl M0210, NEB), 100 μM deoxycytidine triphosphate–Cy3, 5 mM deoxy-ribonucleoside triphosphate (dNTP) mix, 50 mM NaCl, 10 mM tris-HCl, 10 mM MgCl 2 , and 1 mM dithiothreitol (DTT) (pH 7.9).

Techniques: Ligation, Comparison, Sequencing